Fig. 6
From: SIRT1 activation promotes energy homeostasis and reprograms liver cancer metabolism
SCIC2.1 indirectly activates SIRT6 and rescues cells from metabolic stress. a Western blot analysis of SIRT6, PUMA, p21, and Bax performed in HepG2 cells treated with SCIC2.1 and Ex-527 at the indicated concentrations and time points. Heat map showing band quantification of protein expression. b SIRT6 mRNA fold expression evaluated after treatment with SCIC2.1 and Ex-527 at the indicated time points. c CETSA assay performed on HepG2 cells treated with SCIC2.1 (25 µM) and SIRT6 protein expression was analyzed. Heat map shows quantification of SIRT6 bands determined by ImageJ software. Statistical significance was calculated using the student t-test or one-way ANOVA using GraphPad Prism 9.4.0, and statistical significance is expressed as *p-value < 0.05, nsp-value > 0.05 vs control. Error bars represent the standard deviation (SD) of three biological replicates