Fig. 4

SIRT1 activation by SCIC2.1 promotes ATP production and antioxidant response upon glucose starvation. Mitochondrial respiratory parameters following glucose deprivation and bioenergetic parameters associated with SCIC2.1 treatment, and oxygen consumption rate (OCR) of HepG2 cells: a Glucose (25 mM) exposure followed by the treatment with SCIC2.1. b Glucose (5 mM) exposure followed by treatment with SCIC2.1. Histograms show the different parameters (ATP Production, Maximal Respiration, Spare Respiratory Capacity). c Western blot analysis of FOXO3a, NOX2, UCP2, and SOD2 performed in HepG2 cells treated with SCIC2.1 and Ex-527 at the indicated concentrations and time points. Heat map showing band quantification of protein expression determined by ImageJ software. Error bars represent the SD of three biological replicates. Statistical significance was calculated using the student t-test or one-way ANOVA using GraphPad Prism 9.4.0, and statistical significance is expressed as *p-value < 0.05, **p-value < 0.01, and ***p-value < 0.001 vs control.