Fig. 2

SCIC2.1 increases SIRT1 expression upon glucose deprivation and binds to SIRT1. a Western blot analysis of SIRT1 expression in HepG2 cells treated with SCIC2 (50 µM), SCIC2.1 (25 µM), and Ex-527 (5 µM) for 12 h and 24 h under different concentrations of glucose. Heat map showing quantification of SIRT1 bands determined by ImageJ software. b CETSA assay performed on HepG2 cells treated with SCIC2.1 at 25 µM and the western blot analyses showing SIRT1 protein expression. Heat map showing quantification of SIRT1 bands determined by ImageJ software. c SIRT1 protein expression on HepG2 cells treated with SCIC2.1 (25 µM) and Ex-527 (5 µM) at the indicated time points under low-glucose deprivation. Heat map showing band quantification of SIRT1 protein expression determined by ImageJ software. d SIRT1 mRNA fold expression in HepG2 cells after treatment with SCIC2.1 and Ex-527 for the indicated times. Error bars represent the SD of three biological replicates. Statistical significance was calculated using the student t-test or one-way ANOVA using GraphPad Prism 9.4.0, and statistical significance is expressed as *p-value < 0.05, ***pvalue < 0.001, ****p-value < 0.0001 vs control