Fig. 1

SIRT1 activation does not affect cell cycle progression or cell proliferation and rescues cells from genotoxic stress. a Effects of SIRT1 activators on cell cycle progression of HepG2 cells treated with SRT1720 (5 µM), SCIC2 (50 µM), and SCIC2.1 (25 µM) for 24 h. b Percentage of cells in pre-G1 phase. c and d MTT assay on HepG2 and HaCaT cell lines treated with different concentrations of SCIC2.1 for 24 h and 48 h. e and f Western blot analysis on HepG2 and AC16 cells pretreated with the genotoxic drug DOXO (0.5 µM) for 12 h, and then with Ex-527 (5 µM), SRT1720 (5 µM), and SCIC2 and SCIC2.1 (50 µM and 25 µM, respectively) for 6 h. Heat map showing quantification of p53/p53K382ac bands determined by ImageJ software. Error bars represent the standard deviation (SD) of three biological replicates