Fig. 3

Differences between subsets of malignant cells with and without angiogenesis. A Single cell map showing eleven malignant cell subclusters. B UMAP plot of 14,151 cells from eight patient samples. C Tumor cell subclusters composition according to the patients. D Scatter plot showing the distribution of tumor cells in angiogenic and non-angiogenic breast cancer. E–F Violin plots and UMAP figures showing malignant cell-related markers and their distribution in malignant cell subgroups. G UMAP series of single-cell maps of BRCA_AGR3, BRCA_SRGN and BRCA_PPP1R1B. H Immunofluorescence imaging of AGR3, SRGN, PPP1R1B (green) and EpCAM (red) in three breast cancer tissue samples. The nuclei were counterstained with DAPI. Scale bar, 20 µm. I Representative images of the IHC of key genes between breast cancer and normal breast tissues available in the HPA database. J Volcano plot showing differences in gene expression genes in eleven tumor cell subclusters