Fig. 5
RNA-seq identified that AKR1B10 facilitates the Warburg metabolism by regulating the expression of glycolytic enzymes. A KEGG pathways enrichment analysis of downregulated differential genes. B Volcano plot shows the differential genes and the downregulated genes LDHA and LDHB were annotated. C, D The results of qPCR (C) and western blotting (D) indicating the mRNA and protein levels of LDHA in indicated cells, n = 3. E Cellular concentrations of lactate in indicted groups, n = 3. F IHC staining of AKR1B10 and LDHA in brain tumors isolated from mice after intracranial injection with BrM3-shNC or BrM3-shAKR1B10 cells. Scar bar, 100 µM. n = 3. G Representative images of AKR1B10 and LDHA staining in the metastasectomy surgical specimens from lung cancer BM patients. Scar bar, 100 µM. H Scatter diagram showing the correlation between the IRS scores of AKR1B10 and LDHA in surgical samples of 29 lung cancer patients with brain metastasis. (*p < 0.05, ***p < 0.001, ****p < 0.0001, PC9-NC, PC9 transfected with negative control plasmid; PC9-OE, PC9 transfected with AKR1B10 plasmid; shNC, PC9-BrM3 cells transfected with negative control shRNA; sh1, PC9-BrM3 cells transfected with AKR1B10-targeted shRNA vector 1; sh2, PC9-BrM3 cells transfected with AKR1B10-targeted shRNA vector 2; shAKR1B10, PC9-BrM3 cells transfected with AKR1B10-targeted shRNA vector 1)