Fig. 7

Rutaecarpine induces differentiation of TNBC in vivo. A. The growth curves of xenograft tumors derived from 4T1 cells. Mice were subjected to daily treatments with PBS or rutaecarpine. Unpaired Student’s t-test; * p < 0.05. Mean ± SEM. B. The 4T1 tumors removed from mice in each group are shown. C. Statistical analysis of the volume of the dissected tumors at end points. Unpaired Student’s t-test; * p < 0.05. Mean ± SEM. D. HE staining for the tumor slides. E. Proportion analysis for the tumor slides with luminal structure. F. Statistical analysis of the luminal-like area of the tumor slides. Unpaired Student’s t-test; ** p < 0.01. Mean ± SEM. G. IF staining of KRT18 and CD44 for the tumor slides. H. Statistical analysis of the KRT18 positive area of the tumor slides. Unpaired Student’s t-test; ** p < 0.01. Mean ± SEM. I. IF staining of VIM for the tumor slides. J. Statistical analysis of the VIM positive area of the tumor slides. Unpaired Student’s t-test; ** p < 0.01. Mean ± SEM. K. Working model for the mechanism of rutaecarpine induced differentiation of TNBC cells in 3D culture