Fig. 6

Inhibition of FH induces differentiation of TNBC cells through elevated ROS. A. Quantification of fumarase activity of MDA-MB-231 cell lysates under different treatments using a fumarase kit. An FH inhibitor was used as a positive control. One-way ANOVA; **** p < 0.0001. B. Spheroid formation from MDA-MB-231 and BT549 cells following treatment with DMSO, FH inhibitor, and NAC. C. Quantification of circularity and Feret’s diameter for spheroids formed by MDA-MB-231 and BT549 cells. One-way ANOVA; **p < 0.01; ****p < 0.0001. D. Western-blotting showing a reduction in FH in protein level with the knockdown of shRNA of FH in MDA-MB-231 cells. E. Spheroid formation from MDA-MB-231 cells with, and without the knockdown of FH. F. Quantification of circularity and Feret’s diameter for spheroids formed by MDA-MB-231 cells with, and without the knockdown of FH. Unpaired Student’s t-test; **** p < 0.0001. G.TPM analysis of FH, KRT18 and GATA3 genes in tumors from luminal and TNBC patients based on TCGA database. Unpaired Student’s t-test; *** p < 0.001; **** p < 0.0001. H. Relapse-free survival (RFS) classified by the FH transcription levels in breast cancer patients. Log-rank (Mantel-Cox) test; **** p < 0.0001