Fig. 1

Morphological screening of TNBC spheroids identifies that rutaecarpine promotes differentiation in 3D culture. A. Heat map for the basal-like and luminal genes of breast cancer cell lines based on CCLE database. B. Spheroid formation from MDA-MB-231, BT549, MCF7 and T47D cells. C. Quantification of circularity for spheroids formed by MDA-MB-231, BT549, MCF7 and T47D cells. One-way ANOVA; **** p < 0.0001. D. A cutoff value that best discriminated between groups with high or low circularity with respect to differentiation status was determined using the maximal Youden’s index. Chi-square test. E. Schematic for morphological screening to identify natural products that promote differentiation of MDA-MB-231 cells in 3D culture. F. Plot showing the efficiency of natural products in regulating the circularity of MDA-MB-231 spheroids in 3D culture. The top three natural products and DMSO are highlighted