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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Decreased cyclooxygenase-2 associated with impaired megakaryopoiesis and thrombopoiesis in primary immune thrombocytopenia

Fig. 4

The impacts of COX-2 inhibitor celecoxib on megakaryopoiesis and thrombopoiesis in bone marrow derived megakaryocytes. Single-cell suspension of bone marrow from mice were cultured and induced to differentiate into megakaryocytes under different concentrations of COX-2 inhibitor celecoxib in vitro. A The relative count of CD41+CD61+ megakaryocytes in bone marrow cells. Flow cytometry dot plots showed the percentages and gates for CD41+CD61+ megakaryocytes in bone marrow cells. B, C Percentages of CD62P+ cells (B) and Annexin V+ apoptotic cells (C) in CD41+CD61+ megakaryocytes. Flow cytometry dot plots showed the percentages and gates CD41+CD61+ megakaryocytes. D Percentages of polyploid cells in CD41+CD61+ megakaryocytes. Histogram plots showed the percentages and gates for polyploid cells (N > 4) in CD41+CD61+ megakaryocytes. E Relative count of CD41+ platelet granules. F Relative mRNA expression of megakaryopoiesis and thrombopoiesis related genes in megakaryocytes. *P < 0.05, **P < 0.01, ***P < 0.001

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Journal of Translational Medicine

ISSN: 1479-5876

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