Fig. 5

GAA suppresses LRPPRC and OXPHOS complex synthesis in OC cells in vitro and in vivo. A Silencing of LRPPRC via specific shRNA. B Western blot of the levels of OXPHOS-related proteins in A2780 cells with LRPPRC knockdown. C RT-PCR analysis of OXPHOS-related gene levels in A2780 cells with LRPPRC knockdown. D Cell spheroidization assay of A2780 cells with LRPPRC knockdown. E–G Image showing tumor inhibition by LRPPRC. Tumor weights and volumes were measured. H Western blot of LRPPRC levels in cisplatin (DDP)-resistant and cancer stem cells. I RT-PCR detection of LRPPRC levels in cancer stem cells. J RT-PCR detection of OXPHOS-related genes in cancer stem cells. K Western blot of OXPHOS-related proteins in A2780 and SKOV3 cells treated with GAA. L, M RT-PCR analysis of levels of OXPHOS-related genes in A2780 and SKOV3 cells treated with GAA. N IHC results of the staining patterns of LRPPRC. O-Q Western blot of protein levels of ATP5, NDUFB8, UQCRC2 and COX I in different regions of mice