Fig. 6

T-96 inhibited STAT1-CXCL9/CXCL10 signaling by targeting JAK2 in IFN-γ stimulated keratinocytes. T-96 downregulated the CXCR3 expression on CD8⁺ T cells and blocked chemotaxis of CXCR3⁺CD8⁺ cells. A The mRNA and protein expression of STAT1 upon JAK2 knockdown. B The mRNA and secreted proteins of CXCL9 upon JAK2 knockdown. C The mRNA and secreted proteins of CXCL10 upon JAK2 knockdown. D STAT1 mRNA and protein expression upon JAK2 overexpression. E CXCL9 mRNA expression and secreted proteins upon JAK2 overexpression. F CXCL10 mRNA expression and secreted proteins upon JAK2 overexpression. G, H CD8⁺ T cells were cultured in the presence of IL-2 and stimulated with T-96 or Tofa for 48 h. Representative flow cytometric histograms of CXCR3 are shown on left, and the frequency analysis is on right. I–K The migration of CXCR3⁺CD8⁺ and in 3 h responding to the culture supernatants with indicated treatments in NHEKs. *P < .05, **P < .01, ***P < .001, ****P < .0001. ns not significant