Fig. 5

T-96 decreased the expression of CXCL9/10 via JAK2-STAT1 pathway in IFN-γ treated keratinocytes. A, B IC₅₀ value and cell viability of T-96 in NHEKs. Statistical analysis was performed relative to the untreated group. C The GO analysis of the binding proteins of T-96 in IFN-γ-stimulated NHEKs. D Docking analysis of T-96 (chartreuse stick) with JAK2 kinase domain (purple, PDB: 5UT0) (top) and the solvent accessible surface area (bottom). E Interactions were colored by type. F Representative histogram of p-JAK2. G The mRNA and protein expression of STAT1. H Immunofluorescence staining of STAT1 and p-STAT1. Scale bar = 10 μm. I, J The expression of mRNA and secreted proteins of CXCL9/10. *P < .05, ****P < .0001, ns not significant