Fig. 3
From: Pharmacological inhibition of demethylzeylasteral on JAK-STAT signaling ameliorates vitiligo
T-96 inhibited JAK3-STAT5 pathway in CD8+ T cells. A The structure of T-96Bio. B The enriched GO biological process analysis of binding proteins with T-96 Bio in CD8+ T cells. C Molecular docking analysis of T-96 (chartreuse stick) interacting JAK3 kinase domain (brown, PDB: 5TOZ) at the top and the solvent accessible surface area at bottom. D Interactions were colored by type. E, F The expression of phosphorylated JAK3 (p-JAK3) at 15 min and phosphorylated STAT5 (p-STAT5) at 30 min were presented as representative plots (left) and the mean fluorescence intensity (MFI) statical analysis (right). G, H The protein expression of JAK3 and p-JAK3 at 15 min and the ratios of p-JAK3/JAK3. I, J The protein expression of STAT5 and p-STAT5 at 30 min and the ratios of p-STAT5/ STAT5. **P < 0.01, ***P < 0.001, ****P < 0.0001