Fig. 2

T-96 suppressed the proliferation, activation, and function of CD8⁺ T cells. A Structure of T-96. B, C CD8⁺ T cells were cultured under anti-CD3/CD28 Abs, IL-2 and T-96 for indicated dose for IC₅₀ value and cell viability. Statistical analysis was performed relative to the untreated group. D CFSE-labeled CD8⁺ T cells were treated with T-96, Tofa, or both in the presence of IL-2 and anti-CD3/CD28 beads. Representative histograms of proliferation (left) and statistical graph (right). E, F Flow cytometry for the expression of CD69, IFN-γ, GzmB and PRF, representative examples (left), and frequency analysis (right). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns not significant