Fig. 5
From: Improving the cytotoxicity of immunotoxins by reducing the affinity of the antibody in acidic pH
The lysosome degradation and related free toxin release of 32A9 scFv-mPE24 were sensitively affected by chloroquine treatment. A Western blot to detect the stability of internalized immunotoxins in A431-GPC3 cells with or without lysosomal inhibitor treatment. B Statistical analysis of (A). Blue: 32A9 scFv-mE24, red: 42A1 scFv-mPE24. Values represent the mean ± SD, n = 3 individual tests, with *P < 0.05 (paired Student’s t-test). C Western blot to detect free toxin accumulation after immunotoxin internalization into A431-GPC3 cells treated with or without lysosomal inhibitors. D Statistical analysis of (C). Blue: 32A9 scFv-mE24, red: 42A1 scFv-mPE24. Values represent the mean ± SD, n = 3 individual tests, with *P < 0.05 (paired Student’s t-test)