Fig. 9

The alterative expression of some key proteins related to cell apoptosis and DNA damage in LS174T cells after treatment with ZBH-01, CPT-11, and SN38 (50 nmol/l, 24 h), respectively. A, B The result of western blot showed that ZBH-01 is more efficient than CPT-11 and SN38 in regulating the expression of representative proteins. C Histogram of statistical analysis showed the active caspase-3 level in LS174T cells after treatment with ZBH-01, CPT-11, and SN38 (50 nmol/l, 24 h), respectively. D Representative graph showed the active caspase-3 percentage detected by flow cytometry in LS174T cells. ANOVA & two-tailed t-test, n = 3