Fig. 4

ST6GALNAC4 might regulate TGFBR2 expression by elevated glycosylation level in HCC. A heatmap of hierarchical clustering by O-glycosylation state samples. B Enrichment analysis using the KEGG database. C Co-localization of ST6GALNAC4 and TGFBR2 in infected HCCLM3 and MHCC97H cells by immunofluorescence. scale bar, 5 \(\mu\)m; magnification, ×2000. D ST6GALNAC4 antibodies pulled down TGFBR2 protein in coIP experiments. E TGFBR2 antibodies pulled down ST6GALNAC4 protein in coIP experiments. F Effect of ST6GALNAC4 knockdown on TGFBR2,SMAD2/3, pSMAD2, pSMAD3, N-cadherin, E-cadherin, by western blotting, \(\beta\)-tubulin were used as internal standards. G Effect of ST6GALNAC4 overexpression on TGFBR2, SMAD2/3, pSMAD2, pSMAD3, N-cadherin, E-cadherin by western blotting, \(\beta\)-tubulin were used as internal standards. H Effect of TGFB1 Protein and Benzyl-\(\alpha\)-GalNAc in ST6GALNAC4 overexpression cells on SMAD2/3, pSMAD2, pSMAD3 by western blotting, \(\beta\)-tubulin were used as internal standards. I Effect of ST6GALNAC4 knockdown on TGFBR2 and O-GalNAcylation by immunofluorescence. scale bar, 5 \(\mu\)m; magnification, ×2000