Fig. 5

Exogenous ISG15 also reduces the expression of glycosylated PD-L1. A Western blot and RT-qPCR were used to detect the expression of different molecular weights of PD-L1 at concentrations of 0 μg, 1 μg, 1.5 μg, 2 μg, and 4 μg of exogenous ISG15 in A549 and H1299. B H1299 or A549 treated with 4 μg or 0.8 μg of exogenous ISG15, the changes of PD-L1 with different molecular weights (left panel) and mRNA level (right panel) in 0–48 h.The data are shown as the mean ± SD. Ns means no significance. *P < 0.05, **P < 0.01, ***P < 0.001