Fig. 2

ISG15 promotes the infiltration of immune cells in vivo. A Western blot analysis of ISG15 in LLC with over-expressed ISG15 lentiviruses. B The bar graph exhibited the tumor weight of wild-type (WT) and over-expressed ISG15 (Lv-ISG15) LLC cells in immunocompetent C57BL/6 mice. C General views of the lungs and HE staining remove from the mice in WT and LvSG15 groups. Black arrows represent images of metastasis in the lungs. Magnification, ×100, ×400. D Survival curves demonstrating the WT and Lv-ISG15 groups of C57BL/6 mice during one experimental cycle (30 days). E The curve graph exhibited the tumor volume formation of WT and Lv-ISG15 LLC cells in C57BL/6 mice. F Representative images of tumors from C57BL/6 mice with HE and immunohistochemical staining of CD31, KI67, CD3, CD4, and CD8. Scale bars 100 μm. Magnification, ×400. G Populations of tumor-infiltrating CD3⁺ T cells and relative ratio of CD4⁺IFN-γ⁺ and CD8⁺GzmB⁺ cells in CD3⁺ TILs in WT and Lv-ISG15 groups detected by flow cytometry. H Populations of CD4⁺ and CD8⁺ T cells in spleen from the mice in WT and Lv-ISG15 groups detected by flow cytometry. The data are shown as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001