Fig. 2
From: Pharmacologic inhibition of IL11/STAT3 signaling increases MHC-I expression and T cell infiltration
IL11 exerts pro-tumor effect through decreasing CD8+ T infiltration. A Uniform Manifold Approximation and Projection (UMAP) of single-cell sequencing data GSE144735. Cells were grouped and colored in 6 clusters using Seurat. Expression and distribution of IL11+ cells were highlighted. B Percentage and average expression of IL11 in 6 cell types in GSE144735. C Representative images of MC38 subcutaneous tumors in WT and Il11−/− mice (n = 6 mice/group). D Tumor volume (cm3) of MC38 subcutaneous tumors in WT and Il11−/− mice (n = 6 mice/group). ***P < 0.001, two-way ANOVA. E Tumor weight of MC38 subcutaneous tumors in WT and Il11−/− mice (n = 6 mice/group). *P < 0.05, t test. F, G Immunofluorescence and cell counts of CD8 (yellow) and DAPI for nuclei (blue) of MC38 subcutaneous tumors in WT and Il11−/− mice (n = 6 mice/group). Scale bar, 50 μm. *P < 0.05, t test. H Ki-67 staining of MC38 subcutaneous tumors in WT and Il11−/− mice. Scale bar, 50 μm. I Representative images of MC38 subcutaneous tumors in CD8α-depleted WT and Il11−/− mice (n = 6 mice/group). J Tumor volume (cm3) of MC38 subcutaneous tumors in CD8α-depleted WT and Il11−/− mice (n = 6 mice/group). ns, no significance, two-way ANOVA. K Tumor weight of MC38 subcutaneous tumors in CD8α-depleted WT and Il11−/− mice (n = 6 mice/group). ns, no significance, t test