Fig. 3
The RNA sequencing and network pharmacology approach identified differentially expressed genes related to overall survival (OS). A The strategy of next-generation RNA sequencing in kaempferol-treated and negative control EC cells. B Differentially expressed genes (DEGs) between kaempferol-treated and negative control EC cells (Ishikawa and HEC-1-A). Yellow: upregulated differentially expressed genes; Blue: downregulated differentially expressed genes. C A total of 129 overlap genes for DEGs between the kaempferol-treated and negative control EC cells of Ishikawa (blue background) and HEC-1-A (red background). D Differential types of DEGs were identified between the kaempferol-treated and negative control in Ishikawa (ER-positive) and HEC-1-A (ER-negative) cells. E Flowchart for screening endometrial cancer-related genes, kaempferol-related genes, and the related pathway. F A total of 3 genes HSD17B1, CYP1B1, and CYP1A1 were identified in three gene sets, including 169 kaempferol-related genes in silico (red background), 129 kaempferol-related genes in vitro (yellow background), and 1181 endometrial cancer-related genes (purple background). G 67 potential protein-protein interactions were identified. According to the MCODE score, color brightness indicated the strength of the association, with brighter colors indicating a stronger association