Fig. 3
From: PHF6 recruits BPTF to promote HIF-dependent pathway and progression in YAP-high breast cancer
PHF6 recruits BPTF to activate HIF target genes in breast cancer cells. A The ChIP-qPCR assay was conducted with PHF6 antibody in parental and HIF-DKO MCF-7 cells which were exposed to 20% or 1% O2 for 24 h. B The ChIP-qPCR assay was conducted with HIF-1α antibody in control and doxycycline (DOX)-inducible PHF6 knockdown cells which were exposed to 20% or 1% O2 for 24 h. PHF6 proteins were shown by western blotting assay in the indicated groups. C The Co-Immunoprecipitation (Co-IP) assay showing the endogeous interactions between PHF6 and NURF subunits (BPTF, SNF2L, RBBP4). D The ChIP-qPCR assays using BPTF antiboy or IgG control in WT and PHF6-KO MCF-7 cells that were exposed to 20% or 1% O2 for 24 h. E The HIF luciferase reporter activity was detected in MCF-7 cells derived from the indicated groups that were cultured in 20% or 1% O2 for 24 h. F The RT-qPCR analysis of mRNAs of HIF targets and non-HIF target UBLCP1 in shCtrl and shBPTF MCF-7 cells that were exposed to 20% or 1% O2 for 24 h. *P < 0.05, **P < 0.01, ***P < 0.001