Fig. 1
From: PHF6 recruits BPTF to promote HIF-dependent pathway and progression in YAP-high breast cancer
Identification of PHF6 as a required epigenetic regulator for Bca cell growth and progression. A The MTT assay revealing the siRNA KD of 13 candidate PHF family genes and their effects on the growth of MCF-7 cells. Quantitative data shown are representative of 5 experiments. B CCK-8 assays were further used to compare the cell growth abilities in control and shPHF6 groups at the indicated time points. Western blotting assay showed the PHF6 proteins in control and PHF6-KD groups. C Western blotting assay showed the overexpression of PHF6 in Bca cells. D PHF6 overexpression promotes the colony formation abilities in Bca cells. Quantification data was shown on the right in the indicated groups. E The colony formation ability of cells was abolished with PHF6 deficiency, and rescued by ectopic overexpression of PHF6. F, G Assessment of migration and invasion abilities in control and PHF6-overexpressing MCF-7 (F) and MDA-MB-231 (G) cells. Quantification data was shown on the below. H Assessment of migration and invasion abilities in parental control and PHF6-deficient BT-549 cells. Quantification data was shown on the below. *P < 0.05, **P < 0.01, ***P < 0.001