Fig. 2
From: Aa-Z2 triggers ROS-induced apoptosis of osteosarcoma by targeting PDK-1
![Fig. 2](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12967-022-03862-1/MediaObjects/12967_2022_3862_Fig2_HTML.png)
Aa-Z2 suppressed colony formation ability and induced cell apoptosis, along with increased levels of ROS in OS cells. A, B Colony formation assay for Aa-Z2-treated 143B and HOS cells. C, D Transmission electron microscopy for apoptotic bodies of 0.8 μM Aa-Z2-treated 143B and HOS cells. E, F DCFH-DA assay for ROS levels in Aa-Z2-treated 143B and HOS cells. G, H Flow cytometry detection of apoptosis when 143B and HOS cells were treated with Aa-Z2 & NAC. I, J Flow cytometry detection of G2/M phase cells when 143B and HOS cells were treated with Aa-Z2 & NAC. Mean ± SD (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)