Fig. 2
From: Targeting epigenetic features in clear cell sarcomas based on patient-derived cell lines
In vitro characterization and electron micrographs of isolated MUG Lucifer cell lines. Morphology of MUG Lucifer prim cells in 200× magnification using light microscopy. Scale bars represent 100 µm (A). Morphology of MUG Lucifer met cells in 200× magnification using light microscopy. Scale bars represent 100 µm (B). IC50 determination of MUG Lucifer cell lines treated with TKI crizotinib. Concentrations of crizotinib between 0.01 and 100 µM were used for cell viability assessment. Obtained values are normalized to the vehicle control (1% DMSO). IC50 was calculated using GraphPad Prism 9. Error indicators are the positive and negative standard deviations based on biological replicates (n = 3) (C). Cell cycle profiles of MUG Lucifer prim and MUG Lucifer met analysis by ModFit LT™ 5.0.9 (D). Scanning electron micrographs of MUG Lucifer prim and MUG Lucifer met cells; E and F show MUG Lucifer prim cells with the extended surface (asterisk), scale bar represents 1 µm. Micrograph F indicates at higher magnification the close network of these extensions (asterisk), scale bar represents 200 nm. Micrograph G and H show the MUG Lucifer met cells with similar surface, scale bar represents 2 µm; in micrograph H the dimension of these extension is clearly visible (asterisk), scale bar represents 1 µm. Transmission electron micrographs of MUG Lucifer prim (I). Looking closer, cells show a centrally located nucleus (N), a cluster of mitochondria (m) spread through the whole cell in micrograph J. Endoplasmatic reticulum (ER) next to mitochondria, small vesicles within these extensions (V) in micrograph K. In micrograph L autophagosomes (A) within the cells and extensions (asterisk) with different kinds of vesicles (V) are depicted at higher magnification