Fig. 3

ALA inhibited activation of glycolysis in bleomycin-induced pulmonary fibrosis. A Immunohistochemical staining to determine the localization and expression of HK2 and PFKFB3. Bar: 50 μm. B Protein levels of PFKFB3 and HK2 in lung tissues. C, D Co-localization of PFKFB3 and HK2 with collagen I in lung tissues. Original magnification: 200× or 100 pixels. Data are presented as mean ± SEM. *P < 0.05 versus vehicle group; ^#P < 0.05 versus BLM group