Table 3 EMT-associated proteins with enhanced expression in OVCAR5 CBPR cells
Accession ID | Gene names | Protein namesa | Fold change (CBPR/parental) | p value | Involvement in EMT |
|---|---|---|---|---|---|
P17301 | ITGA2 | Integrin alpha-2 | 3.61 | 0.0001 | - Ectopic expression of ITGA2 induced VIM and CDH2 expression while downregulated CDH1 [23] |
Q15582 | TGFBI | Transforming growth factor-beta-induced protein ig-h3 | 3.13 | 0.0135 | - Enhanced expression of TGFBI stimulates invasive progression in vitro and correlates with poor prognoses in cancer patients [25, 29] |
P15121 | AKR1B1 | Aldose reductase | 3.00 | 0.0017 | - High expression of correlates with aggressive and invasion tumor phenotype [74] |
P06756 | ITGAV | Integrin alpha-V; Integrin alpha-V heavy chain; Integrin alpha-V light chain | 2.52 | 0.0014 | - Downregulation of ITGAV via the anti-tumor miR-9-3p inactivates EMT, suppresses proliferation and metastasis in vitro [11] - ITGAV interference inhibited tumor growth through abolishing TGFβ1-SMAD signaling [96] |
Q13501 | SQSTM1 | Sequestosome-1 | 2.12 | 0.0293 | - Ectopic expression of SQSTM1 led to morphological and molecular changes in vitro, including spindle-shaped cells, low CDH1 expression, high levels of CDH2, VIM and SNAI1 [97] |
P56199 | ITGA1 | Integrin alpha-1 | 2.10 | 0.0478 | - ITGA1-collagen binding induced cell spreading and shift towards a mesenchymal morphology [71] |
O94808 | GFPT2 | Glutamine–fructose-6-phosphate aminotransferase [isomerizing] 2 | 2.07 | 0.0086 | - Immediate-early gene product induced by NF-κB in mesenchymal cells - GFPT2 silencing reduced migration and invasion in ovarian cancer cells, HEY and SKOV3 [77] |
P21333 | FLNA | Filamin-A | 2.01 | 0.0028 | - Interacts with SMAD2 to promote EMT via SMAD2 target genes, Snai1 and MMP9 [98] |