Fig. 6

Lumican knockdown decreased ECM deposition in an LPS-induced ALI mouse model. Lumican mRNA levels in the lung tissue (A), and BALF levels of fibronectin (B) and COL3A1 (C) were assessed in mice intratracheally administrated with PBS or AAV-packed scrambled shRNA or lumican shRNA with or without LPS challenge at 24 h (n = 4–5 per group). Values are expressed as the mean and standard deviation. (D) Representative lung sections stained with immunofluorescence stain which labels DAPI in blue, lumican in green and fibronectin in red in mouse lung sections. Scale bar, 50 μm. (E) Representative lung sections stained with Masson’s trichrome stain in mice. The significance of between-group differences was examined using two-way analysis of variance followed by Tukey's multiple comparisons test to analyse the means of multiple samples. p < 0.05 was considered statistically significant. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.0001. AAV adeno-associated virus, ALI acute lung injury, BALF bronchoalveolar lavage fluid, COL3A1 alpha-1 type III collagen, DAPI 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, ECM extracellular matrix, LPS lipopolysaccharides, PBS phosphate-buffered saline, TNF-α tumour necrosis factor-alpha