Fig. 4

piRNA-14633 increases m6A levels and elevates METTL14 mRNA expression. a, b EpiQuik M6A RNA Methylation Quantification Kit (colorimetric method) was used to detect m6A levels in Siha and Caski cells treated with 50 nM mi-14633 or 50 nM in-14633. c, d Real-time PCR was performed to detect METTL3, METTL14, WTAP, ALKBH5 and FTO mRNA levels in Caski cells. e, f Real-time PCR was performed to determine METTL3, METTL14, WTAP, ALKBH5 and FTO mRNA levels in Siha cells. *P < 0.05 vs. mi-NC group. ^#P < 0.05 vs. in-NC group. mi-14633 piRNA-14633 mimic, in-14633 piRNA-14633 inhibitor