Fig. 6
Elimination of E. coli heals bone resorption in a mouse model of myeloma in complete remission. A C57BL/KaLwRij mice were intravenous injected with 5TGM1 (5 × 105 cells/mouse) and E. coli cells (1 × 104 cells/mouse). After 4 weeks, 1 mg/kg bortezomib and 2 mg/kg melphalan with or without Amp (100 mg/kg) were injected intraperitoneally into the mice (3 times a week) for 2 weeks. B The expression of E. coli 16S rRNA in mice bone marrow aspirates. Histomorphometric analysis of BV/TV (C), OS/BS (D), Ob.S/BS (E), ES/BS (F) and Oc.S/BS (G). Data are means ± SD (n = 5 mice/group, three replicate studies). *P < 0.05. P values were determined using Student’s t test. H Depiction of signaling pathways involved in the E. coli-mediated suppression of osteoblastogenesis and activation of osteoclastogenesis