Table 6 Isolation method characteristics
Manufacturer | Roche | ID-Solution | Taigen | Perkin Elmer |
|---|---|---|---|---|
Robot | MagNA Pure 24 System | IDEAL | LabTurbo 24 AB | Chemagic 360D |
A | B | C | D | |
Product | MagNA Pure 24 Total NA Isolation Kit | IDXTRACT-MAG | Virus combo kit 24C-LVX480-1000 | NextPrep-Mag cf DNA isolation kit |
Type | Bead based | Bead based | Column | Bead based |
Sample volume (mL) | 2–4 | 0.5–5 | 0.3–3 | 0.5–10 |
Elution volume (µL) | 30–200 | 30–120 (adaptable) | 30–200 | 30–100 |
Level of automation | Full | Semi | Full | Semi |
Manual pre processing | Reconstitute the PK and incubation (5 min at room temperature), addition of beads + buffers (incubation with vigorous shaking for 30 min at 56 °C). Adaptable distribution of buffers and elution reagents | Wash preparation: addition of EtOH | Add beads, add elution reagent in a bar or tube, reconstitute the Proteinase K | |
Hands-on-time (min) | 0 | 45 | 5 | 20 |
Automated runtime (min) | 90 | 45 | 70 | 70 |
Total runtime (min) | 90 | 90 | 75 | 90 |
Samples | 24 | 24 | 24 | 12 |
Cost of the instrument | + | + | + | ++ |
Cost of consumables | ++ | + | ++ | + |
Dimensions (cm) | ||||
Height × width × length | 77.5 × 76.5 × 89.9 | 37 × 57 × 72 | 87 × 74 × 65 | 90 × 82 × 90 |