Fig. 5
![Fig. 5](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12967-020-02539-x/MediaObjects/12967_2020_2539_Fig5_HTML.png)
Faecal microbiota from CAD patients induce systematic immune activation and IFN-γ response. a, c Volcano plot showing pairwise comparisons of differential expression of genes (DEGs) between Con (n = 3) versus CAD (n = 3) mice in the liver and ileum, respectively. Dots represent genes that are significantly different (|Log2(foldchange)| > 2, adjust P value < 0.05). b, d PCA scatter plot of DEGs in the liver and ileum between Con (n = 3) and CAD (n = 3) mice, respectively. e, f) Results of gene ortholog (GO) enrichment analysis in the liver and ileum, respectively. Bar indicate gene counts for each GO term. g Serum inflammatory factors including IL-1β and TNF-α in Con and CAD group. n = 11 or 12 per group. h Serum LPS level. n = 11 or 12 per group. i CD4+IFN-γ+ T cells in the spleen (left) and percentages of CD4+IFN-γ+ T cells within CD4+ T cells (right). *P < 0.05, **P < 0.01, Mann–Whitney U test. Con mice, n = 9, CAD mice, n = 10