Fig. 7

Examples of integrated neutrophil chemotaxis devices combined with isolation units. a The donut-shape microfluidic device. Chemoattractant is introduced into the focal chemotaxis chambers (FCCs), and a gradient is generated along the migration channels towards the FCCs. The whole blood is put into the whole blood loading chamber (WBLC). The red blood cells are prevented by the RBC filtration comb and only neutrophils can migrate out of the whole blood and accumulate in the FCCs. This is adapted from Jones, C. N., Hoang, A. N., Dimisko, L., Hamza, B., Martel, J., Irimia, D. Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood. J. Vis. Exp. (88), e51215, https://0-doi-org.brum.beds.ac.uk/10.3791/51215 (2014). b The all-on-chip device for neutrophil chemotaxis analysis. The device consists of the cell docking barrier channel (4 µm) and the gradient generation channel (60 µm). This is adapted from Yang, K., Wu, J., Zhu, L., Liu, Y., Zhang, M., Lin, F. An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood. J. Vis. Exp. (124), e55615, https://0-doi-org.brum.beds.ac.uk/10.3791/55615 (2017); c Overview of the KOALA platform. Compared with the traditional methods like transwell assay that take many hours to complete all the processes, this platform significantly reduces the experiment time. (Figure reproduced from Ref. [43])