Fig. 1

Identification of adipose-derived MSCs and peritoneal macrophages. Cultivated Passage 3 MSCs stained positive for CD29-APC and CD90-APC and negative for CD34-FITC, CD45-FITC and CD11b-FITC via flow cytometry. Negative control was also presented. The target cells we chose were in the FSC-Height and SSC-Height quadrant to separate cell debris (a). MSC morphology was observed via light microscopy, and MSCs were stained by Oil Red O or Alizarin Red to indicate differentiation into adipocytes or osteoblasts (b). Macrophage morphology was observed via light microscopy, and more than 99% of the cultured macrophages stained positive for CD11b and CD68 via flow cytometry (c). Scale bar: 100 μm