Table 1 Primer oligonucleotide sequences (forward and reverse) used in RT-qPCR
From: Dermal tissue remodeling and non-osmotic sodium storage in kidney patients
Primer | Forward sequence | Reverse sequence |
|---|---|---|
A. Inflammation | ||
 CCL2 | 5′-AGACTAACCCAGAAACATCC-3′ | 5′-ATTGATTGCATCTGGCTG-3′ |
 VCAM | 5′-TCCTGAGCTTCTCGTGCTCTATT-3′ | 5′-TGACCCCTTCATGTTGGCTT-3′ |
B. Fibrosis | ||
 COL1A1 | 5′-GGGATTCCCTGGACCTAAAG-3′ | 5′-GGAACACCTCGCTCTCCA-3′ |
C. Lymphangiogenesis | ||
 VEGFC | 5′-CTGGCTCAGGAAGATTTTATG-3′ | 5′-TGTTTTTACAGACACACTGG-3′ |
 PDPN | 5′-AAGATGGTTTGTCAACAGTG-3′ | 5′-GTACCTTCCCGACATTTTTC-3′ |
D. Proteoglycan related | ||
 VCAN | 5′-CCAGTGTGAACTTGATTTTG-3′ | 5′-CAACATAACTTGGAAGGCAG-3′ |
 NDST1 | 5′-CGTGACGCGACCTAGCGA-3′ | 5′-TCATAGGTGGAGTGATTTGACTGG-3′ |
 HS6ST1 | 5′-AGGAAGTTCTACTAACATCACC-3 | 5′-CCCATCACACATATGCAAC-3′ |
 HSPE | 5′-CCTTGCTATCCGACACCTTT-3′ | 5′-GGCTGACAGGCCCAATTTA -3′ |
 CHYSY1 | 5′-AGACTTTCAGCAAAATCCAG-3′ | 5′-GTTTGAGAGAAAGGACAAGG-3′ |
 HAS1 | 5′-TCCACTGTGTATCCTGCATC-3′ | 5′-CCCCAAAAGTATCCTGCATC -3′ |
 HAS2 | 5′-GATGCATTGTGAGAGGTTTC-3′ | 5′-CCGTTTGGATAAACTGGTAG-3′ |
 HAS3 | 5′-CTTGAAGATTAATGTAGGATGACAGGCT-3′ | 5′-AAAGTTGACGACCACAGTGCAA-3′ |
 UST | 5′-GAACGTGAATGAAAACTTCC-3′ | 5′-TCTGGGTCTTTGTAGATACTG-3′ |
 CHST11 | 5′-TATTTCCAAATCATGCGGAG-3′ | 5′-ATTGGGTTGTAGAGTTCCTG-3′ |
E. Housekeeping | ||
 β-Actin | 5′-CCAACCGCGAGAAGATGA-3′ | 5′-CCAGAGGCGTACAGGGATAG-3′ |