Table 4 Summarized results of in vitro testing for heparin impact on cfDNA assay
From: Autoimmune disorders but not heparin are associated with cell-free fetal DNA test failure
Experiment | Sample | Heparin | Molecule | Concentration IU/mL | Library prep (nM) | Reads (M) | Fetal fraction (%) | Z-score 21 | Z-score 18 | Z-score 13 |
|---|---|---|---|---|---|---|---|---|---|---|
1 | NPP | No | – | – | 26.16 | 20.1 | 11.7 | − 0.78 | − 1.55 | 0.97 |
1 | NPP | No | – | – | 31.01 | 17.0 | 10.91 | 0.92 | − 0.85 | 0.01 |
1 | NPP | No | – | – | 32.29 | 16.0 | 10.32 | − 0.72 | 0.00 | − 0.42 |
1 | NPP | No | – | – | 30.79 | 18.9 | 10.03 | − 0.44 | − 0.08 | − 0.48 |
1 | Entire flow cell | No | – | – | 31.10 | 18.0 | 10.20 | 0.00 | 0.04 | 0.01 |
1 | NPP | Yes | Enoxaparin | 0.6 | 27.05 | 18.8 | 10.80 | 0.36 | 0.80 | − 0.01 |
1 | NPP | Yes | Enoxaparin | 1.2 | 34.37 | 18.5 | 11.10 | 1.51 | 0.20 | 0.24 |
1 | NPP | Yes | Enoxaparin | 2.2.44 | 23.89 | 18.7 | 11.90 | 0.31 | − 0.58 | 0.45 |
1 | NPP | No | – | – | 22.40 | 19.9 | 11.25 | − 0.75 | − 0.49 | − 0.76 |
1 | NPP | No | – | – | 26.28 | 19.7 | 10.53 | − 0.34 | 0.22 | − 0.20 |
1 | NPP | No | – | – | 29.70 | 17.0 | 11.08 | − 0.72 | − 1.07 | − 0.84 |
1 | NPP | No | – | – | 32.84 | 19.2 | 11.34 | − 0.35 | 0.04 | − 0.46 |
1 | Entire flow cell | No | – | – | 30.98 | 18.8 | 9.80 | 0.02 | 0.00 | 0.02 |
1 | NPP | Yes | Tinzaparin | 0.43 | 27.05 | 21.6 | 9.40 | − 1.21 | 0.42 | 1.49 |
1 | NPP | Yes | Tinzaparin | 0.87 | 34.37 | 21.2 | 9.50 | − 0.17 | 0.04 | − 1.43 |
1 | NPP | Yes | Tinzaparin | 1.74 | 23.89 | 19.7 | 10.00 | − 0.34 | − 0.02 | 0.24 |
2 | NPP | No | – | – | 38.95 | 18.6 | 8.97 | 0.67 | − 0.02 | − 2.15 |
2 | NPP | No | – | – | 37.99 | 18.5 | 10.32 | − 1.17 | 1.16 | − 0.16 |
2 | NPP | No | – | – | 35.89 | 17.4 | 11.86 | − 1.34 | − 0.06 | − 0.05 |
2 | NPP | No | – | – | 36.63 | 18.2 | 11.85 | − 0.10 | − 0.72 | − 0.73 |
2 | Entire flow cell | No | – | – | 33.40 | 18.2 | 10.35 | 0.06 | 0.08 | 0.06 |
2 | NPP | Yes | Enoxaparin | 0.6 | 37.98 | 18.9 | 11.30 | 0.68 | − 0.26 | 1.25 |
2 | NPP | Yes | Enoxaparin | 1.2 | 32.51 | 19.9 | 10.40 | 0.15 | 0.15 | − 0.61 |
2 | NPP | Yes | Enoxaparin | 2.2.44 | 36.92 | 20.3 | 11.50 | 0.35 | − 0.37 | − 1.03 |
2 | NPP | No | – | – | 22.33 | 19.8 | 10.26 | 0.43 | 0.66 | 0.56 |
2 | NPP | No | – | – | 24.38 | 17.7 | 10.25 | − 0.15 | − 0.76 | 0.51 |
2 | NPP | No | – | – | 26.56 | 17.5 | 9.93 | − 0.10 | 0.21 | 1.21 |
2 | NPP | No | – | – | 28.83 | 18.0 | 10.44 | 0.70 | − 0.47 | − 0.99 |
2 | Entire flow cell | No | – | – | 29.00 | 18.2 | 10.30 | − 0.01 | 0.03 | − 0.07 |
2 | NPP | Yes | Tinzaparin | 0.43 | 32.24 | 22.2 | 10.50 | 0.37 | 0.92 | 0.68 |
2 | NPP | Yes | Tinzaparin | 0.87 | 33.72 | 19.2 | 9.50 | 1.03 | 0.59 | 0.56 |
2 | NPP | Yes | Tinzaparin | 1.1.7474 | 32.06 | 20.5 | 10.30 | 0.19 | 1.12 | 0.54 |