Fig. 5

Anti-B7-H3 mAb suppresses Th cell differentiation co-cultured with macrophages transfected by anti-miR-29c. Macrophages transfected with anti-miR-29c were harvested and were co-cultured with CD4⁺ T cells (1 × 10⁵ cells/ml) in the presence of control IgG (20 µg/ml) or anti-B7-H3 mAbs (20 µg/ml). ROR-γt and GATA-3 expression were decreased in CD4⁺ T cells compared to control IgG group. The level of IL-4 in supernatant was also decreased compared to control IgG group. However, an addition of anti-B7-H3 in the cultures had no effect on expression of IL-17. NS not significant; *P < 0.01; ***P < 0.001. Data are shown as mean ± STD