Fig. 8

Effects of rhACE2 on renal oxidative stress levels in the Ang II-infused ApoEKO mice. a, b The real-time PCR analysis revealed mRNA expression of the NADPH oxidase subunits NOX2 (a) and NOX4 (b) in mice kidneys (n = 4–6). GAPDH was used as an endogenous control. c–e Representative dihydroethidium fluorescence images (c), relative fluorescence values (d) and lucigenin-enhanced chemiluminescence assay (e) exhibited the superoxide generation and NADPH oxidase activity in mice kidneys. n = 4–5. AU arbitrary units, ApoE apolipoprotein E, ACE2 angiotensin-converting enzyme 2, KO knockout, rhACE2 human recombinant ACE2, Ang II angiotensin II, PEG-SOD polyethylene glycol-conjugated superoxide dismutase, DPI diphenylene iodonium chloride (NADPH oxidase inhibitor). *P < 0.05; **P < 0.01 compared with the ApoEKO control group; ^## P < 0.01, compared with the ApoEKO + Ang II group.