Figure 3From: Clinically-relevant consecutive treatment with isoproterenol and adenosine protects the failing heart against ischaemia and reperfusion Lactate dehydrogenase (LDH) activity and infarct size in hearts of the Series 1. The experiments were carried out on Control (n = 7) and Iso/Ade groups with 0 (n = 9), 5 (n = 9), and 10 (n = 9) min washout periods. Panel A - LDH activity in the effluent perfusate during 30 min reperfusion. Differences between Control and all three Iso/Ade groups (measured for the corresponding data points) were statistically significant (P < 0.05) at all data points. There was no significant difference in LDH activity between the Iso/Ade groups. Panel B – Infarct size. Mean ± SEM (columns) and representative images of the corresponding heart slices stained with 2,3,5-triphenyltetrazolium chloride (TTC). ** P < 0.01, *** P < 0.001 vs. Control; # P < 0.05 vs. Iso/Ade group with zero min washout period.Back to article page