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Table 2 Healthy Donor Sera Analysis

From: Multiplex serum biomarker assessments: technical and biostatistical issues

Sample

Date

Drawn

Assay

Date

IL-4

pg/mL

IL-6

pg/mL

IL-8

pg/mL

IL-10

pg/mL

TNF-α

pg/mL

IFN-g

pg/mL

GM-CSF

pg/mL

IP-10

pg/mL

MIG

pg/mL

MCP-1

pg/mL

Healthy donor 1

12/14/2009

2/2/2010

57

43

25

21

16

44

90

28

143

324

  

2/2/2010

58

48

22

25

16

49

94

30

143

319

  

5/13/2010

50

39

19

22

13

31

87

25

103

263

  

5/13/2010

52

29

18

17

11

29

78

25

100

254

  

8/11/2010

36

42

11

25

< 10

20

40

31

71

30

  

8/11/2010

29

39

11

20

< 10

16

39

29

64

31

Healthy donor 2

12/16/2009

2/2/2010

57

61

44

41

20

160

179

31

96

632

  

2/2/2010

59

59

40

42

18

163

151

31

105

589

  

5/13/2010

44

48

29

31

15

109

129

28

70

529

  

5/13/2010

41

49

34

33

15

109

128

27

69

521

  

8/11/2010

82

80

63

37

42

100

84

40

151

160

  

8/11/2010

67

91

68

39

34

105

81

35

165

138

Healthy donor 3

12/17/2009

2/2/2010

17

< 8

18

< 14

< 10

< 12

< 15

23

20

977

  

2/2/2010

21

< 8

19

< 14

< 10

< 12

< 15

23

13

921

  

5/13/2010

22

< 8

21

< 14

< 10

< 12

< 15

22

< 12

803

  

5/13/2010

20

< 8

18

< 14

< 10

< 12

< 15

23

< 12

763

  

8/11/2010

24

< 3

18

< 5

< 10

< 5

< 15

19

11

241

  

8/11/2010

32

< 3

21

< 5

< 10

< 5

< 15

23

16

258

Healthy donor 4

12/18/2009

2/2/2010

111

29

88

51

50

189

253

39

196

577

  

2/2/2010

121

31

90

51

56

212

262

37

216

579

  

5/13/2010

81

20

66

38

39

147

211

30

128

468

  

5/13/2010

76

21

60

36

33

142

201

30

133

440

  

8/11/2010

232

48

160

65

90

137

173

49

277

171

  

8/11/2010

222

46

167

68

92

141

167

48

276

183

Healthy donor 5

12/21/2009

2/2/2010

12

9

33

< 14

< 10

18

33

20

32

194

  

2/2/2010

< 5

< 8

30

< 14

< 10

14

25

20

13

192

  

5/13/2010

5

< 8

34

< 14

< 10

14

< 15

19

< 12

177

  

5/13/2010

< 5

< 8

32

< 14

< 10

< 12

< 15

20

12

174

  

8/11/2010

7

7

26

8

< 10

> 5

< 15

25

20

16

  

8/11/2010

12

12

33

10

< 10

< 5

23

27

20

17

Healthy donor 6

12/21/2009

2/2/2010

19

8

24

< 14

< 10

< 12

37

22

50

496

  

2/2/2010

< 5

< 8

28

< 14

< 10

< 12

17

22

37

558

  

5/13/2010

19

< 8

15

< 14

< 10

< 12

18

19

40

434

  

5/13/2010

10

< 8

< 12

< 14

< 10

< 12

18

18

36

413

  

8/11/2010

12

12

47

12

14

7

21

27

48

111

  

8/11/2010

18

11

45

13

13

7

21

28

51

108

Healthy donor 7

12/22/2009

2/2/2010

16

< 8

38

< 14

< 10

35

56

19

66

1040

  

2/2/2010

17

9

40

< 14

10

35

62

19

66

1019

  

5/13/2010

19

< 8

35

< 14

< 10

40

51

17

50

843

  

5/13/2010

20

< 8

41

< 14

< 10

33

51

18

53

848

  

8/11/2010

13

6

28

28

< 10

19

37

21

24

245

  

8/11/2010

16

10

30

41

11

25

49

24

24

246

Healthy donor 8

12/23/2009

2/2/2010

54

15

39

29

17

82

135

40

188

926

  

2/2/2010

58

17

33

29

17

78

123

42

188

934

  

5/13/2010

64

15

37

32

21

76

144

38

160

815

  

5/13/2010

65

15

35

33

20

72

129

36

160

742

  

8/11/2010

23

< 3

10

8

< 10

9

22

34

71

144

  

8/11/2010

32

6

14

14

< 10

21

36

34

96

130

Healthy donor 9

12/24/2009

2/2/2010

< 5

9

17

< 14

< 10

< 12

< 15

21

13

969

  

2/2/2010

< 5

8

15

< 14

< 10

< 12

< 15

20

20

928

  

5/13/2010

< 5

< 8

13

< 14

< 10

< 12

< 15

17

< 12

784

  

5/13/2010

< 5

< 8

14

< 14

< 10

< 12

< 15

19

< 12

813

  

8/11/2010

8

11

19

9

< 10

6

< 15

29

20

332

  

8/11/2010

7

10

17

8

< 10

< 5

< 15

26

20

331

Healthy donor 10

12/28/2009

2/2/2010

< 5

8

< 12

< 14

< 10

< 12

< 15

37

13

1034

  

2/2/2010

< 5

< 8

< 12

< 14

< 10

16

< 15

37

13

990

  

5/13/2010

< 5

< 8

< 12

< 14

< 10

< 12

< 15

34

< 12

845

  

5/13/2010

< 5

< 8

< 12

< 14

< 10

< 12

< 15

36

< 12

802

  

8/11/2010

< 5

4

< 3

< 5

< 10

< 5

< 15

57

6

374

  

8/11/2010

5

5

< 3

< 5

< 10

8

< 15

59

11

385