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Table 1 Spheroid size and cellular characteristics as a function of age in spheroids.

From: Establishment and characterization of multicellular spheroids from a human glioma cell line; Implications for tumor therapy

Age of spheroids in days

Radius (μm)

Av. volume × 10-6(μm3)

Avg. No. of cells/spheroid

Avg. Cellular Diameter (μm)

Cell packing density

Viability (%)

Plating Efficiency (%)

7

93 ± 5

3244

1176 ± 39

13 ± 1

0.36

98 ± 1.4

65 ± 0.6

14

148 ± 10

13300

2222 ± 47

7 ± 1(20%); 14 ± 1

0.17

78 ± 1.9

63 ± 0.2

21

157 ± 11

15896

4236 ± 63

10 ± 1 (25%); 16 ± 1 (63%); 24 ± 2 (12%)

0.27

77 ± 1.7

45 ± 0.2

28

242 ± 19

57891

8975 ± 78

19 ± 2

0.16

70 ± 3.2

25 ± 0.1

  1. Note: All values are mean ± SD of 20–50 observations from each experiment (radius, surface area, volume, Number of cells/spheroid and average size of cells) or 6 observations (viability and plating efficiency) from three experiments.