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Table 1 Spheroid size and cellular characteristics as a function of age in spheroids.

From: Establishment and characterization of multicellular spheroids from a human glioma cell line; Implications for tumor therapy

Age of spheroids in days Radius (μm) Av. volume × 10-6(μm3) Avg. No. of cells/spheroid Avg. Cellular Diameter (μm) Cell packing density Viability (%) Plating Efficiency (%)
7 93 ± 5 3244 1176 ± 39 13 ± 1 0.36 98 ± 1.4 65 ± 0.6
14 148 ± 10 13300 2222 ± 47 7 ± 1(20%); 14 ± 1 0.17 78 ± 1.9 63 ± 0.2
21 157 ± 11 15896 4236 ± 63 10 ± 1 (25%); 16 ± 1 (63%); 24 ± 2 (12%) 0.27 77 ± 1.7 45 ± 0.2
28 242 ± 19 57891 8975 ± 78 19 ± 2 0.16 70 ± 3.2 25 ± 0.1
  1. Note: All values are mean ± SD of 20–50 observations from each experiment (radius, surface area, volume, Number of cells/spheroid and average size of cells) or 6 observations (viability and plating efficiency) from three experiments.