Fig. 8
Ultrastructure representations obtained by transmission electron microscopy (TEM) on AC16 human cardiomyocytes exposed to ISO and PSELT, alone or in combination. A Control group (scale bar: 2 μm, magnification × 4000); B ISO group (scale bar: 2 μm, magnification × 4000) and B1 (scale bar: 1 μm, magnification × 8000; C ISO + PSELT group (scale bar: 2 μm, magnification × 4000) and C1 (scale bar: 1 μm, magnification × 8000; D PSELT group (scale bar: 2 μm, magnification × 4000); E quantitative TEM analysis showing the number of Golgi stacks per cell that was determined by a counting grid method from 10 different electron microscope images for each sample; F quantitative TEM analysis of mitochondrial cristae number based on the quantification of the cristae per mitochondrial section from different mitochondria. Only mitochondria with intact and completely visible borders have been included in the analysis