Fig. 5

Effects of ISO and PSELT on SELENOT expression and role of endogenous protein on cell hypertrophy in H9c2 cardiomyocytes. A Western blot analysis of SELENOT expression in H9c2 cells exposed to vehicle (saline, NaCl 0.9%) indicated as a CTRL, isoproterenol (ISO) (100 µM), ISO + PSELT and PSELT (5 nM). Histograms represent the ratio of densitometric analysis of protein:loading control. Data are expressed as the mean ± SEM (n = 3 independent experiments). Significant differences were detected by one-way ANOVA and Newman-Keuls multiple comparison test. *p < 0.05. B Morphological staining of H9c2 cells transfected with Negative control si-RNA (NC si-RNA) or Selenoprotein T si-RNA (SELENOT si-RNA) for 36 h and then treated with or without ISO (100 µM) for additional 48 h. Scale bars: 25 μm. Cell surface area (%) was quantified by ImageJ. Data are expressed as mean ± SEM from three independent experiments. Significant differences were detected by one-way ANOVA and Newman-Keuls multiple comparison test. **p < 0.01; ***p < 0.001. C Representative Western blot analysis of SELENOT in H9c2 cardiomyocytes transfected with NC si-RNA or SELENOT si-RNA for 36 h. Histograms represent the ratio of densitometric analysis of protein:loading control. Data are expressed as the mean ± SEM (n = 3 independent experiments). Significant differences were detected by t-test. *p < 0.05