Fig. 1

Clinical timeline and tissue specimens. A, Melanoma course, occurrence of irAEs, and specimens collected from patient MA-6. The top timeline shows tissue collections; the middle timeline shows melanoma status and systemic therapies (not including focal radiotherapy); the bottom timeline shows the irAEs and their pharmacologic management. Not included in the bottom timeline is the onset of grade 2 adrenal insufficiency of clinically unclear etiology, treated with hydrocortisone replacement. IFN-a, adjuvant interferon alfa. B, Tissue specimens from patient MA-6 that were used in this study. Biopsy and surgical specimens were collected pre-mortem, others were collected at autopsy. Green boxes, assays performed; black boxes, assays not done. IHC immunohistochemistry; met, metastasis, qRT-PCR quantitative real-time reverse transcription polymerase chain reaction, TCRseq T-cell receptor sequencing