Table 1 Summary of all gene-specific primer pairs used for semi-quantitative RT-PCR
Target gene | Sequence (5ʹ → 3ʹ) | PCR product size | |
|---|---|---|---|
ACTB | Forward: | TTTGAGACCTTCAACACCC | 209Â bp |
Reverse: | AGGATCTTCATGAGGTAGTC | ||
ARID1A | Forward: | CCCCTCAATGACCTCCAGTA | 159Â bp |
Reverse: | CTGGAAATCCCTGATGTGCT | ||
EGF | Forward: | GAATCATGGCTGTACTCTTG | 317Â bp |
Reverse: | GGTCATACCCAGGAAAGC | ||
FGF1 | Forward: | ACAGTGGATGGGACAAG | 287Â bp |
Reverse: | TAGTGAGTCCGAGGACC | ||
FGF2 | Forward: | CACTTCAAGGACCCCAA | 310Â bp |
Reverse: | CAGTGCCACATACCAACTG | ||
GAPDH | Forward: | CATCACTGCCACCCAGAAGA | 291Â bp |
Reverse: | GTGTAGCCCAGGATGCCTTT | ||
PDGFA | Forward: | TGGAGATAGACTCCGTAGG | 162Â bp |
Reverse: | TGACCGTCCTGGTCTTG | ||
TGFβ1 | Forward: | AGTCAAGAAAAGTCCGCACAG | 180 bp |
Reverse: | CTGAGGTAGCGCCAGGAATC | ||
VEGFA | Forward: | CGAAGTGGTGAAGTTCATG | 240Â bp |
Reverse: | CCTATGTGCTGGCCTTG | ||