Fig. 9

Effects of secreted products derived from the siRNA-transfected MDCK cells on ECs tube formation. Conditioned medium (CM) was harvested after 24-incubation of the siRNA-transfected MDCK cells in serum-free medium. ECs tube formation was determined by incubating EA.hy926 cells for 24 h in matrigel-coated wells containing CM derived from the siRNA-transfected MDCK cells. A Micrographs of the capillary/mesh-like tubes in each condition taken by an inverted phase-contrast light microscope. B–C Numbers of nodes (junctional part) and meshes (hollow part) of the ECs tubes were measured from 10 random fields per each sample using the angiogenesis analyzer for ImageJ software (https://imagej.nih.gov/ij/). The dots represent individual data points derived from three biological replicates in three independent experiments, whereas the error bar represents mean ± SD of each group. Only significant p values are labeled