Fig. 8

Effects of secreted products derived from the siRNA-transfected MDCK cells on ECs migration. Conditioned medium (CM) was harvested after 24-incubation of the siRNA-transfected MDCK cells in serum-free medium. ECs migration was determined by allowing EA.hy926 cells in the upper chamber with serum-free medium to migrate into in the lower chamber containing CM from each condition. After 24h incubation, the migrated ECs were stained with a fluorescence dye and imaged under the fluorescence microscope. A Micrographs of the immunofluorescence-stained migrated ECs in each condition. B Numbers of the migrated ECs were counted from 15 random fields per each sample. The dots represent individual data points derived from three biological replicates in three independent experiments, whereas the error bar represents mean ± SD of each group. Only significant p values are labeled