Fig. 1

Schematic diagram for analytical methods in this study. A ARID1A protein complex in MDCK renal cells was isolated from the whole cell lysate by IP with anti-ARID1A antibody. In parallel, the sample immunoprecipitated with isotype IgG served as the control for background (non-specific) subtraction. The immunoprecipitated proteins were resolved by SDS-PAGE and subjected to in-gel tryptic digestion followed by identification by nanoLC-ESI-LTQ-Orbitrap MS/MS and bioinformatic analyses to predict protein–protein interactions network and functional enrichment. B The MS/MS data were validated by IP and reciprocal IP followed by immunoblotting. C To study functions, single and double knockdowns of ARID1A and its interactor by siRNA were performed in MDCK cells. RNA was extracted and expression levels of angiogenesis-related genes were determined by semi-quantitative RT-PCR. Finally, effects of secreted products derived from the siRNA-transfected MDCK cells on angiogenesis features (including cell proliferation, migration and tube formation) of EA.hy926 ECs were examined