Fig. 4

WGX50 alleviates DOX-induced cardiomyocytes injury by counteracting ferroptosis in HL-1 cells. A Cell viability assay of HL-1 cardiomyocytes when treated with 0, 0.5, 1, 2, 5, 10, 50, 100, 200 µM of WGX50 for 24 h. B HL-1 cells were cultured with 2 µM DOX in combination with or without 10 μM Fer-1 or with WGX50 at various concentrations (2, 10, 50 μM) for 3, 6, 12, and 24 h. Cell viability was measured by CCK8 method. C–G The levels of ANP, BNP, CK-MB, cTnT, and Fe²⁺ in HL-1 cardiomyocytes were determined by RT-qPCR and assay kits as available procedures across groups (B). H Western blot bands show relative expression level of GPX4 protein in HL-1 cardiomyocytes across groups as indicated in (B). The histogram illustrates the quantitative data of GPX4 protein after β-actin normalization. Values are expressed as mean ± SD from three individual experiments (n = 3). Values are expressed as mean ± SD from three individual experiments (n = 3). *, **, and *** respectively means p < 0.05, p < 0.01, and p < 0.001, “ns” means not statistically significant